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fit msd curves option  (Oxford Instruments)
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Oxford Instruments fit msd curves option
FIGURE 5. Heterogeneous migratory behavior of thymocytes expressing ccr9a and ccr9a/b in the double-transgenic (ccr9a:h2b-gfp;ccr9b:tagRFP) fish at 11 dpf. (A) Average cell speed of individual thymocytes over a 30-min period. Number of tracked cells for ccr9a and ccr9a/b are 653 and 334, re- spectively. (B) Trajectories of ccr9a (top panel) and ccr9a/b cells (bottom panel) with average cell speed less than (blue) or greater than (yellow) 3 mm/min. <t>(C)</t> <t>Displacement</t> length of ccr9a and ccr9a/b cells with average cell speed less than (blue) or greater than (yellow) than 3 mm/min. Error bars show SE in mean. (D) Straightness of individual thymocytes with average cell increased to 3 mm/min. (E) Trajectories of ccr9a (left panel) and ccr9a/b cells (right panel). Lateral view of thymus. Color-coded trajectories indicate the straightness values according to (D). <t>MSD</t> graphs (F) and track plots of ccr9a/b (G) and ccr9a (H) cells with different straightness values. In track plots, the cell trajectories were aligned for the starting position. The red line in (F) represents the linear regression (R) analysis for each plot. ***p , 0.001.
Fit Msd Curves Option, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 5. Heterogeneous migratory behavior of thymocytes expressing ccr9a and ccr9a/b in the double-transgenic (ccr9a:h2b-gfp;ccr9b:tagRFP) fish at 11 dpf. (A) Average cell speed of individual thymocytes over a 30-min period. Number of tracked cells for ccr9a and ccr9a/b are 653 and 334, re- spectively. (B) Trajectories of ccr9a (top panel) and ccr9a/b cells (bottom panel) with average cell speed less than (blue) or greater than (yellow) 3 mm/min. (C) Displacement length of ccr9a and ccr9a/b cells with average cell speed less than (blue) or greater than (yellow) than 3 mm/min. Error bars show SE in mean. (D) Straightness of individual thymocytes with average cell increased to 3 mm/min. (E) Trajectories of ccr9a (left panel) and ccr9a/b cells (right panel). Lateral view of thymus. Color-coded trajectories indicate the straightness values according to (D). MSD graphs (F) and track plots of ccr9a/b (G) and ccr9a (H) cells with different straightness values. In track plots, the cell trajectories were aligned for the starting position. The red line in (F) represents the linear regression (R) analysis for each plot. ***p , 0.001.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Noninvasive In Toto Imaging of the Thymus Reveals Heterogeneous Migratory Behavior of Developing T Cells.

doi: 10.4049/jimmunol.1500361

Figure Lengend Snippet: FIGURE 5. Heterogeneous migratory behavior of thymocytes expressing ccr9a and ccr9a/b in the double-transgenic (ccr9a:h2b-gfp;ccr9b:tagRFP) fish at 11 dpf. (A) Average cell speed of individual thymocytes over a 30-min period. Number of tracked cells for ccr9a and ccr9a/b are 653 and 334, re- spectively. (B) Trajectories of ccr9a (top panel) and ccr9a/b cells (bottom panel) with average cell speed less than (blue) or greater than (yellow) 3 mm/min. (C) Displacement length of ccr9a and ccr9a/b cells with average cell speed less than (blue) or greater than (yellow) than 3 mm/min. Error bars show SE in mean. (D) Straightness of individual thymocytes with average cell increased to 3 mm/min. (E) Trajectories of ccr9a (left panel) and ccr9a/b cells (right panel). Lateral view of thymus. Color-coded trajectories indicate the straightness values according to (D). MSD graphs (F) and track plots of ccr9a/b (G) and ccr9a (H) cells with different straightness values. In track plots, the cell trajectories were aligned for the starting position. The red line in (F) represents the linear regression (R) analysis for each plot. ***p , 0.001.

Article Snippet: The squared displacement values were calculated using Imaris Fit MSD curves option.

Techniques: Expressing, Transgenic Assay